Journal: Nature Communications
Article Title: Diet-induced RKIP downregulation disrupts PC/PE-ER homeostasis to drive MASLD
doi: 10.1038/s41467-025-65982-8
Figure Lengend Snippet: a Representative fluorescence images of Bodipy 493/503-stained LDs (left), the percentage of LDs (middle; Fresh groups, n = 6; Veh/PA groups, n = 5) and the mean number of LDs per cell (right, n = 20) in primary hepatocytes from 2 months old RKIP f/f and Alb cre RKIP f/f mice (Fresh cells without plating (Fresh); cells plating 24 hrs with or without PA treatment). Green, Bodipy 493/503; Blue, DAPI; Scale bars, 40 μm. b Mean fluorescent intensity (MFI) of Bodipy 493/503 in mouse primary hepatocytes as described in A ( n = 3), determined by flow cytometry. c, e Representative fluorescence images of LDs, the percentage of LDs (HepG2 groups, n = 3; HeLa groups, n = 5) and the mean number of LDs per cell ( n = 20) in WT and RKIP knockout (KO) HepG2 ( c ) and HeLa cells ( e ) with PA stimulation for 24 hrs. Scale bars, 40 μm. d , f MFI of Bodipy 493/503 in PA-stimulated WT and KO HepG2 ( d ) and HeLa cells ( f ) ( n = 3), determined by flow cytometry. g, h Representative fluorescence images of LDs (left), the percentage of LDs (middle; n = 4) and the mean number of LDs per cell (right, n = 20) in PA (24 hrs) -stimulated WT and KO HepG2 ( g ) and HeLa ( h ) cells transduced with Vector or RKIP-flag lentivirus. Scale bars, 40 μm. Data are presented as mean ± SEM ( a–h ). Each dot represents a technical replicate ( a, c, e, g, h ) or a biological replicate ( b, d, f ). ns, no significance. * p < 0.05, ** p < 0.01, *** p < 0.001. P values were calculated by two-tailed unpaired Student’s t -tests ( a–f ) and one-way ANOVA in ( g, h ). Data are representative of at least two independent experiments. Source data are provided as a Source Data file .
Article Snippet: Golgi was stained by living cell dye Golgi-tracker (Beyotime Biotechnology, C1043) at 4 °C for 30 min. LDs were stained by living cell dye Bodipy 493/503 (4 mM, MedChemExpress, HY-W090090).
Techniques: Fluorescence, Staining, Flow Cytometry, Knock-Out, Transduction, Plasmid Preparation, Two Tailed Test
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